Fig. 2

miR-106a-5p abundance in exosomes from highly metastatic CRC cells. (A) Volcano plot exhibiting the differentially expressed exosomal miRNAs in the GSE115114 and GSE123708. (B) qRT-PCR was performed to detect the expression of four miRNAs in exosomes derived from four CRC cells. (C) The expression of miR-106a-5p in NCM460 and CRC cell lines. (D) The expression of miR-106a-5p in Mφ after treated with PBS, Caco-2 exosomes, HCT 116 exosomes, SW480 exosomes or SW620 exosomes, respectively. E-F. The expression levels of miR-106a-5p in Mφ after incubated with physically (E) or pharmacologically (F) exosome-depleted supernatants compared to those treated with standard supernatant. G. The expression levels of miR-106a-5p were detected in the supernatant of CRC cells following treatment with RNase A or RNase A plus Triton X-100. H. The presence of green fluorescent signals in Mφ after treated with PKH67-labeled exosomes for 24 h. Scale bar = 10 μm. I. Mφ were incubated with exosomes derived from SW620/SW480/HCT 116/Caco-2 transfected with Cy3-labeled miR-106a-5p (red). Scale bar = 10 μm. The data presented herein represent the outcomes of a minimum of three independent experiments and are depicted as the mean ± standard deviation (SD). ns = No significant difference, * P < 0.05, ** P < 0.01, *** P < 0.001