Fig. 6

The loss of JunB contributes to the LDHA downregulation. A GSEA to assess specific enrichment of glycolysis signaling pathway upon RNA-seq (siNAT10 VS siNC). NES, normalized enrichment score; p-value was calculated with a permutation test. B, C qRT-PCR showed the mRNA expression of glycolysis-related gene after NAT10 knockdown. D Identification of transcription factors interacting with LDHA promoter by Pscan (http://159.149.160.88/pscan/) online promoter database. E The motif of JunB binding LDHA promoter, prompted by Pscan. F, G ChIP–qPCR assay to detect the enrichment of JunB at the promoter of the LDHA locus. H-J LDHA expression upon the NAT10 and JunB knockdown, and JunB overexpression. The data are shown as the means ± SDs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001