Fig. 5

NAT10 contributes to the upregulation of ac4C modification in JunB mRNA. A Venn diagram showed the integration results of RNA-seq, IL-4, and IL-13 signaling pathway and TGF-β signaling pathway. B Molecular docking showing the interaction between NAT10 and JunB. C The relative levels of ac4C in JunB were tested by acRIP-qPCR in TNBC cells with NAT10 knockdown or overexpression. D, E The interaction between NAT10 and JunB mRNA was analyzed by RIP-qPCR assay in TNBC cells with NAT10 knockdown or overexpression. F, G mRNA and protein level of JunB upon NAT10 knockdown or overexpression. H, I RNA decay experiment validated JunB mRNA stability upon NAT10 knockdown or overexpression. The data are shown as the means ± SDs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001