Fig. 3

NAT10 function is required for immunosuppressive TME in TNBC. A t-SNE analysis of immune cells in 4T1 tumor, showing the identification of 8 main clusters. Each dot corresponds to a single cell, colored according to the cell clusters. B t-SNE subplot showing individual markers’ expression and distribution. C-E Normalized expression of Granzyme B, PD-1, CD86, and CD206 is shown by the t-SNE plot. F–H Quantification of subgroup changes in T cell and macrophage populations. The data are shown as the means ± SDs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001