Fig. 2

Altered LDB1 expression affects the proliferation of T-ALL cells. A The knockdown levels of LDB1 in cells was verified by WB. B Western blotting analysis showed that LDB1 and GAPDH protein pression in Jurkat, 6 T-CEM and J.gamma1 cells after LDB1 knockdown. C RT-PCR analysis showed that LDB1 and GAPDH protein pression in Jurkat, 6 T-CEM and J.gamma1 cells after LDB1 knockdown. D CCK-8 assay detected the proliferation rate of Jurkat, 6 T-CEM and J.gamma1 cells after LDB1 knockdown. E Colony-forming assay for Jurkat, 6 T-CEM and J.gamma1 cells infected with sh-NC or sh-LDB1#1. F Flow cytometry using Annexin V staining showed that knockdown of LDB1 increased the apoptotic rates of Jurkat, 6 T-CEM and J.gamma1 cell lines. G Knockdown of LDB1 increased the Annexin V⁺375 fraction and promoted apoptosis in Jurkat, 6 T-CEM and J.gamma1 cell lines. H Western blotting analysis showed that the PARP, Cleaved Caspase3 and Cleaved Caspase8 were upregulated in T-ALL cells after LDB1 knockdown, while BCL2 was downregulated