Fig. 9

The inhibition of DNM1L/DRP1-FIS1 axis impeded MRC structure and damaged mitochondrial quality. a and b Detected the MMP and ROS of U87MG after down-regulation of DNM1L/DRP1, the mean fluorescence intensity of positive cells was measured. (n = 6). c The cross-section of the MRC was highlighted in green in the electron microscope images, and a statistical analysis was conducted to determine the extent of MRC occupancy. Scale bars: 0.3 µm. d and e Detected the MMP and ROS of U87MG after overexpression of DNM1L/DRP1, the mean fluorescence intensity of positive cells was measured (n = 6). f The details depicted in the picture align with those presented in (c). g and h Detected the MMP and ROS of U87MG after down-regulation of FIS1, the mean fluorescence intensity of positive cells was measured (n = 6). i The details depicted in the picture align with those presented in (c). j and k Detected the MMP and ROS of U87MG after overexpression of FIS1, the mean fluorescence intensity of positive cells was measured (n = 6). l The details depicted in the picture align with those presented in (c). m and n Detected the MMP and ROS of U87MG after down-regulation of MFF, the mean fluorescence intensity of positive cells was measured. (n = 6). o The details depicted in the picture align with those presented in (c). p and q Detected the MMP and ROS of U87MG after overexpression of MFF, the mean fluorescence intensity of positive cells was measured (n = 6). r The details depicted in the picture align with those presented in (c). The data were presented as means ± SD. P were calculated by unpaired two-tailed t-test (a, b, d, e, g, h, j, k, m, n, p, q) or Mann-Whitney U test (c, f, i, l, o, r)