Fig. 4
The overexpression of DNM1L/DRP1 significantly increased the expression of FIS1 while suppressing MFF. a Western blot, expression level of mitochondria-related proteins in the U87MG when DNM1L/DRP1 was downregulated and overexpressed. b Statistical analysis of Western blot after regulation of DNM1L/DRP1. c Representative images of electron microscopy after down-regulation and overexpression of DNM1L/DRP1 (U87-sh-DNM1L-NC, n = 7; U87-sh-DNM1L, n = 6; U87-OE-DNMIL-NC, n = 7; U87-OE-DNM1L, n = 8). Scale bars: 0.5 µm. d Statistical analysis of the mitochondrial size, density and shape after regulation of DNM1L/DRP1 (U87-sh-DNM1L-NC, n = 118; U87-sh-DNM1L, n = 104; U87-OE-DNMIL-NC, n = 108; U87-OE-DNMIL, n = 199). e Western blot, expressions of mitochondria-related proteins in the U87MG after Mdivi-1 intervention. f Statistical analysis of Western blot after Mdivi-1 intervention in U87MG. g Representative images of electron microscopy after Mdivi-1 intervention, (U87-DMSO, n = 6; U87-Mdivi-1, n = 8). Scale bars: 0.5 µm. h Statistical analysis of the mitochondrial size, density and shape after Mdivi-1 intervention (U87-DMSO, n = 102; U87-Mdivi-1, n = 87). The data were presented as means ± SD. P were calculated by unpaired two-tailed t-test (b, f) or Mann-Whitney U test (d, h)