Fig. 3
Reducing DNM1L/DRP1 hindered HGG progression, while up-regulating DNM1L/DRP1 can promote the progression of HGG. a The CCK-8 assay, detected the proliferation ability of U87MG after regulation of DNM1L/DRP1 (n = 6). b The Transwell assay, detected the migration ability of U87MG after regulation of DNM1L/DRP1 (n = 6). Scale bars: 200 µm. c The Wound Healing assay, detected the migration ability of U87MG after regulation of DNM1L/DRP1 (n = 6). Scale bars: 500 µm. d Glioma cell derived xenograft assay (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. e Representative confocal images of U87MG in sh-DNM1L, sh-DNM1L-NC, OE-DNM1L and OE-DNM1L-NC: DAPI (blue), Ki-67 (red) and merge. Scale bars: 50 µm. Quantitative analysis of % of Ki-67⁺ cells (n = 6). The data were presented as means ± SD. P were calculated by unpaired two-tailed t-test (b, e) or two-way ANOVA (a, c, d)