Fig. 3

Decreased glycolysis cells lead to the development of a high-glucose and low-lactate TME. A Representative western blot analysis results of Ldha and β-actin protein levels in whole cell lysates of shNC, shLDHA-1, shLDHA-2, and shLDHA-3 cells. B ECAR results of shNC and shLDHA cells (n = 5). C Glucose consumption and lactate production by shNC and shLDHA cells (n = 3, 24 h). D Proliferation rates of shNC and shLDHA cells (n = 5). E, F Orthotopic transplantation of shNC and shLDHA cells (n = 5) into (E) BALB/c-nu mice and (F) C57BL/6 mice for 20 days, followed by quantification of tumor weight (one of the BALB/c-nu mice transplanted with shNC cells had died). G–J FCM analysis of (G) the percentages of tumor-infiltrating CD45⁺ cells in shNC and shLDHA tumors (n = 5) of C57BL/6 mice, and the percentages of (H) tumor-infiltrating CD8⁺ T cells, (I) DCs, and (J) MDSCs among the CD45⁺ cells. K–M Representative IHC staining of (K) CD8⁺ cells, (L) CD11c⁺ cells, and (M) CD11b⁺ cells in tumors of C57BL/6 mice. Scale bar, 100 μm. N Relative fold changes of glucose and lactate levels in shNC and shLDHA tumors (n = 5)