Fig. 3

MED1 knockdown inhibits metastatic OSCC cells migration and invasion in vitro. A Cell scratch assay to examine SCC-9 cells migration ability after MED1 knockdown. B Quantitative analysis of the cell migration ratio in (A). n = 3 independent experiments. C Cell scratch assay to examine UPCI-SCC-154 cells migration ability after MED1 knockdown. D Quantitative analysis of the cell migration ratio in (C). n = 3 independent experiments. E Transwell invasion assay to examine SCC-9 cells invasion ability after MED1 knockdown. F Quantitative analysis of the number of invasive cells in (E). n = 3 independent experiments. G Transwell invasion assay to examine UPCI-SCC-154 cells invasion ability after MED1 knockdown. H Quantitative analysis of the number of invasive cells in (G). n = 3 independent experiments. I MMP2 and MMP9 gene expression assessed by qRT-PCR in SCC-9 cells after MED1 knockdown. n = 3 independent experiments. J MMP2 and MMP9 gene expression assessed by qRT-PCR in UPCI-SCC-154 cells after MED1 knockdown. n = 3 independent experiments.Scale bar = 200 µm (10 ×). Bars = means ± SD.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001