Fig. 2

The levels of pERK are reduced in KCH^−/− pancreata. A Schematic representation of LSL-KRas^G12D/+ and Hipk2^flox/flox recombinant alleles. B Representative genotyping analysis for Hipk2^flox and LSL-KRas^G12D alleles of tail (T) and pancreas (P) from Pdx1-Cre;LSL-KRas^G12D/+ (KC) and Pdx1-Cre;LSL-KRas^G12D/+;Hipk2^flox/flox (KCH^−/−) mice. Floxed (2lox) and recombined (1lox) alleles are indicated by arrows. C WB analysis of pERK levels in KC and KCH^−/− pancreata. HSP70 was used as loading control. D Normalized levels of pERK represented as ratio of pERK and total ERK densitometry values. Mean ± Standard Deviation (SD) is reported. Student’s t-test, * P < 0.05. E Representative IHC images for each pERK score. Scale bars are 100 µm. F Representative IHC images of pERK in KC and KCH^−/− pancreata. Scale bars are 200 µm. G Scatter plot bar of pERK score in all KC and KCH^−/− mice. Mean ± SD is reported at the bottom. Mann–Whitney’s test, * P < 0.05