Fig. 7

Targeted inhibition of the WDR5-KMT2A interaction by MM-102 weakens the proliferation, migration, and chemoresistance of CCA. A: The protein levels of ABCB1, MBD2 and WDR5 was examined by western blot in TFK and RBE cells supplemented with MM-102. B: The representative image of wound healing assay to detect the migration of TFK1 and RBE cells added with MM-102. The right panel is the statistical volume. C-D: The proliferation abilities of TFK1 and RBE cells supplemented with MM-102 was detected by CCK-8 assay (C) and plate cloning assay (D). E: IC50 value of TFK1 and RBE cells to cisplatin upon added with MM-102. F: Representative image of liver in primary CCA mice models treated with cisplatin or MM-102. Cis: cisplatin. G: HE staining of the liver in primary CCA mice models treated with cisplatin or MM-102. Cis: cisplatin. H: The ratio of liver weight to body weight in primary CCA mice models. I: IHC staining of Ki-67 in liver of primary CCA mice models. The right panel is the statistical of Ki-67 rate. *P < 0.05, **P < 0.01, ***P < 0.001