Fig. 6

MBD2 activates the expression of ABCB1 through the WDR5-KMT2A complex. A-B: The protein levels of ABCB1 was examined by western blot in WDR5 knockdown and overexpression cells. C: Western blotting was used to detect the effect of KMT2A knockdown in TFK1 and RBE cells. D: The protein levels of ABCB1 was examined by western blot in KMT2A knockdown cells. E-F: The protein levels of ABCB1 was detected by western blot upon overexpression of MBD2 and knockdown of WDR5. G-H: The protein levels of ABCB1 was detected by western blot upon overexpression of MBD2 and knockdown of KMT2A. I: The GEO database indicates binding peaks of MBD2, WDR5, and KMT2A on the ABCB1 gene. The below panel is the schematic diagram of binding peak of ABCB1 and the ChIP-qPCR product (P1, P2, P3, P4). J-M: The ChIP-qPCR was used to examine the binding of MBD2, WDR5 and H3K4me3 to ABCB1 in MBD2 or WDR5 overexpression TFK1 cells. Anti-Flag antibody was used for ChIP-qPCR to detect the enrichment of MBD2-Flag or WDR5-Flag in the binding region; Anti-H3K4me3 antibody was used for ChIP-qPCR to detect the enrichment of H3K4me3 in the binding region. ***P < 0.001