Fig. 5

WDR5 promotes the proliferation, migration and chemoresistance of CCA. A: Western blotting was performed to detect the effect of WDR5 knockdown in TFK1 and RBE cells. B-D: The proliferation abilities of TFK1 and RBE cells with WDR5 knockdown was detected by CCK-8 assay (B and C) and plate cloning assay (D). E: Representative images of wound healing assay used to detect the migration of TFK1 and RBE cells in which WDR5 was knocked down and the statistical volume. F: Tumors of xenograft mice models derived from TFK1 cells with WDR5 knockdown. The image below shows the statistical analysis of the tumor volume and the Ki-67 rate. G: IC50 values of CCA cells in response to cisplatin upon WDR5 knockdown. H: Overexpression of WDR5 in TFK1 and RBE cells was validated by western blotting. I-K: The proliferation abilities of TFK1 and RBE cells overexpressing WDR5 were detected by CCK-8 assay (I and J) and plate cloning assay (K). L: Representative images of wound healing assay in which WDR5 was overexpressed in TFK1 and RBE cells and the statistical volume. M: The tumor of xenograft mice models derived from TFK1 cells with WDR5 overexpression. The image below is the statistical of tumor volume and Ki-67 rate. N: IC50 values of TFK1 and RBE cells response to cisplatin upon WDR5 overexpression. **P < 0.01, ***P < 0.001