Fig. 4

MBD2 directly interacts with WDR5 in CCA cells. A: The gel of the negative control group and MBD2-IP group from 293T cells. The protein samples were separated by SDS-PAGE and stained with Coomassie brilliant blue. The protein masses are shown in the left. B: Co-localization of MBD2 and WDR5 was detected by immunofluorescence. C-E: The interaction of MBD2 with WDR5 was detected by IP in 293T, TFK1 and RBE cells. F: The direct interaction of MBD2 with WDR5 was confirmed by GST pull-down. G: The schematic diagram of the domain organization of MBD2 and its truncated mutants. H: 293T cells was transfected with the full length or the truncated mutants of MBD2. Co-IP was used to detect which of the truncated fragment of MBD2 bound to WDR5. I: IP was used to detect the binding ability between Flag-M1 fragments and WDR5