Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Tumor-associated neutrophils upregulate Nectin2 expression, creating the immunosuppressive microenvironment in pancreatic ductal adenocarcinoma

Fig. 6

ER stress increased in TANs, producing protumor functions. (A). Gene ontology analysis of genes whose expression was upregulated in human TANs compared with original neutrophils. (B) Heatmap of genes involved in the response to unfolded proteins (UPR) between neutrophils and TANs. (C) Western blotting results show ER stress-related proteins (p-ERK and BIP1) in human neutrophils differentiated from HL60 cells and those treated with the supernatant of SUIT-2 cells (TANs) (left panels). Western blotting results identifying ER stress-related proteins (BIP and CHOP) in neutrophils from peripheral blood (PBNs) and orthotopically implanted tumors (TANs) in mice (right panels). β-actin was used as a loading control. (D) Relative expression levels of ER stress-related genes in humans (left panel, 18 S served as an internal control) and mouse (right panel, GAPDH served as an internal control) neutrophils during phenotype switch. (n = 3 per group). (E) Relative expression levels of N2 marker genes in human (TANs) and 4-Phenylbutyric acid (4-PBA)-pretreated TANs (4PBA-TANs). The mRNA expression level of each gene was normalized to fold over 18 S. (n = 3 per group). (F) Western blotting results demonstrating BHLHE40 expression in neutrophils, TANs, and 4PBA-TANs. β-actin was used as a loading control. (G) Relative expression levels of CCL5 mRNA in TANs and 4PBA-TANs. (n = 3 per group). (H-I) Flow cytometry analysis of Nectin2 expression in TANs and 4PBA-TANs (H). The percentage of Nectin2 + neutrophils (I). (J) The migration and invasion of SUIT-2 and MIA PACA-2 cells treated with TAN’CM or 4PBA-TAN’CM, as assessed using transwell assays (n = 3 per group). *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page

Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

Contact us