Fig. 8
From: HSF1 is a prognostic determinant and therapeutic target in intrahepatic cholangiocarcinoma
The HSF1 inhibitor KRIBB11 decreases the mitochondrial bioenergetics in cholangiocarcinoma cell lines. A Cell Mito Stress test profiles of human HuCCT1 and KKU-M156 cell lines treated with 10 µM KRIBB11 and matching DMSO concentration for 24 h. Seahorse XF Cell Mito Stress Tests (Agilent) were performed employing serial injections of oligomycin, FCCP, and Rot/AA; Hoechst 33342 was injected last for nuclei staining required for normalization. OCR was measured in real-time using the Seahorse XF HS mini analyzer. B Changes in basal respiration, maximal respiration and ATP production. Data were background corrected and normalized to the mean fluorescent intensity per well; a factor 105 was applied. Graphs depict the mean ± SEM of two independent experiments each performed in technical triplicates (multiple Mann–Whitney tests; *p < 0.05; **p < 0.01). Abbreviations: OCR, oxidative consumption rate (in pmol/min); FCCP, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; Rot/AA, rotenone/antimycin A