Fig. 5

Triple therapy alters the gene expression profile of tumor-infiltrating immune cells and sophisticated immune regulation. A and B NanoString analysis of the tumor microenvironment. Secondary tumors in the 344SQ-P and 344SQ-R models were harvested at seven days post-XRT, followed by RNA extraction and NanoString immune profiling for 770 immune-related genes. A Heatmaps showing pathway score. Orange indicates high scores; blue indicates low scores. Scores are displayed on the same scale via a Z-transformation. B Specific genes with significant differences in the comparison of XRT + RDB 1462 + anti-PD1 and control in the 344SQ-P and 344SQ-R models. C and D TCR repertoire analysis. Blood samples were collected from the cheek of 3 mice/ group on day 16 in 344SQ-P tumor model. C The Simpson index (sample diversity) and the number of clonotypes for the indicated groups. D) Specific clonotypes were identified as increasing or appearing unique when treated with XRT or combined with RDB 1462