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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Two-polarized roles of transcription factor FOSB in lung cancer progression and prognosis: dependent on p53 status

Fig. 6

FOSB had heterogenous impacts on the tumor biology in a specific transcriptional target-dependent manner in NSCLC cells expressing p53 in variable statuses (A) Effects of PREX1 knockdown on the relative proliferation rate in H1299 (p53-Null) cells with FOSB overexpression, evaluated by the CCK-8 cell proliferation assay; (B) Effects of PREX1 knockdown on the independent viability in H1299 (p53-Null) cells with FOSB overexpression, evaluated by the colony formation assay; (C) Effects of PREX1 knockdown on the migration and invasion capabilities in H1299 (p53-Null) cells with FOSB overexpression, evaluated by the Transwell migration (Without the matrix gel coating) and invasion (With the matrix gel coating) assays; (D) Effects of PREX1 knockdown on the xenograft tumor growth in H1299 (p53-Null) cells with FOSB overexpression, evaluated by the cell line-derived xenograft (CDX) models (Scale bar: 1 cm, n = 3); (E) Effects of PREX1 knockdown on the protein expression levels of FOSB, p53, and Ki-67 in xenograft tumor tissues developed by H1299 (p53-Null) cells with FOSB overexpression, evaluated by the Western Blot with quantitative analysis; (F) Effects of IGFBP5 knockdown on the relative proliferation rate in H1299 (p53-WT) and A549 (p53-WT) cells with FOSB overexpression, assessed by the CCK-8 cell proliferation assay; (G) Effects of IGFBP5 knockdown on the independent viability in H1299 (p53-WT) and A549 (p53-WT) cells with FOSB overexpression, assessed by the colony formation assay; (H) Effects of IGFBP5 knockdown on the migration capability in H1299 (p53-WT) and A549 (p53-WT) cells with FOSB overexpression, assessed by the Transwell migration assay; (I) Effects of IGFBP5 knockdown on the invasion capability in H1299 (p53-WT) and A549 (p53-WT) cells with FOSB overexpression, assessed by the matrix gel-coated Tranwell invasion assay; (J) Effects of IGFBP5 knockdown on the xenograft tumor growth in H1299 (p53-WT) and A549 (p53-WT) cells with FOSB overexpression, assessed by the CDX models (Scale bar: 1 cm, n = 3); (K) Effects of IGFBP5 knockdown on the protein expression levels of FOSB, p53, and Ki-67 in xenograft tumor tissues developed by H1299 (p53-WT) and A549 (p53-WT) cells with FOSB overexpression, assessed by the Western Blot with quantitative analysis; (L) Effects of IGFBP5 knockdown on the cisplatin sensitivity in H1299 (p53-WT) and A549 (p53-WT) cells with FOSB overexpression, assessed by the CCK-8 cytotoxicity assay; (M) Effects of AKR1C3 or ALDH3A1 knockdown on the relative proliferation rate in H1299 (p53-R248Q) and PC-9 (p53-R248Q) cells with FOSB overexpression, estimated by the CCK-8 cell proliferation assay; (N) Effects of AKR1C3 or ALDH3A1 knockdown on the independent viability in H1299 (p53-R248Q) and PC-9 (p53-R248Q) cells with FOSB overexpression, estimated by the colony formation assay; (O) Effects of AKR1C3 or ALDH3A1 knockdown on the migration capability in H1299 (p53-R248Q) and PC-9 (p53-R248Q) cells with FOSB overexpression, estimated by the Transwell migration assay; (P) Effects of AKR1C3 or ALDH3A1 knockdown on the invasion capability in H1299 (p53-R248Q) and PC-9 (p53-R248Q) cells with FOSB overexpression, estimated by the matrix gel-coated Tranwell invasion assay; (Q) Effects of AKR1C3 knockdown on the xenograft tumor growth in H1299 (p53-R248Q) and PC-9 (p53-R248Q) cells with FOSB overexpression, estimated by the CDX models (Scale bar: 1 cm, n = 3); (R) Effects of AKR1C3 knockdown on the protein expression levels of FOSB, p53, and Ki-67 in xenograft tumor tissues developed by H1299 (p53-R248Q) and PC-9 (p53-R248Q) cells with FOSB overexpression, estimated by the Western Blot with quantitative analysis; (S) Effects of AKR1C3 or ALDH3A1 knockdown on the cisplatin sensitivity in H1299 (p53-R248Q) and PC-9 (p53-R248Q) cells with FOSB overexpression, estimated by the CCK-8 cytotoxicity assay. * P < 0.05, ** P < 0.01, *** P < 0.001

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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