Fig. 5

FOSB differentially regulated downstream signaling pathways in a specific transcriptional target-dependent manner in NSCLC cells expressing p53 in variable statuses (A) The abridged general view depicting the signaling pathways associated with tumor progression and prognosis that are respectively mediated by PREX1 (The left panel), IGFBP5 (The middle panel), AKR1C3 and ALDH3A1 (The right panel), summarized from the previous publications issued; (B) Effects of FOSB overexpression on the “PREX1-RAC1-MAPK (ERK)/AKT”, “IGFBP5-p53”, “IGFBP5-MAPK (ERK)/AKT”, and “AKR1C3-MAPK (ERK)/AKT” signaling pathways in H1299 (p53-Null), H1299 (p53-WT), A549 (p53-WT), H1299 (p53-R248Q), and PC-9 (p53-R248Q) cells, detected by the Western Blot; (C) Effects of the siRNA-mediated PREX1 knockdown on the regulation of the PREX1-RAC1-MAPK (ERK)/AKT oncogenic signaling pathways by FOSB in H1299 cells expressing p53-Null, detected by the Western Blot followed with quantitative analyses; (D) Effects of the siRNA-mediated IGFBP5 knockdown on the regulation of the IGFBP5-p53 oncosuppressive signaling pathway and the MAPK (ERK)/AKT oncogenic signaling pathways by FOSB in H1299 and A549 cells expressing p53-WT, detected by the Western Blot followed with quantitative analyses; (E) Effects of the siRNA-mediated AKR1C3 or ALDH3A1 knockdown on the regulation of the AKR1C3/ALDH3A1-MAPK (ERK)/AKT oncogenic signaling pathways by FOSB in H1299 and PC-9 cells expressing p53-R248Q, detected by the Western Blot followed with quantitative analyses. * P < 0.05, ** P < 0.01, *** P < 0.001