Fig. 4

FOSB overexpression induced unique transcriptomic alterations in NSCLC cells expressing p53 in variable statuses (A) The schematic diagram illustrating the transcriptome sequencing process for H1299 cells expressing p53-Null, p53-WT, and p53-R248Q upon the transfection of the FOSB plasmid; (B) The volcano maps of the DEGs (FC > 1.5, P < 0.05) in H1299 cells expressing p53-Null, p53-WT, and p53-R248Q upon the transfection of the FOSB plasmid; (C) The Venn diagram showing the intersection sets among the three up-regulated DEGs sets (P < 0.05) in H1299 cells expressing p53-Null, p53-WT, and p53-R248Q upon the transfection of the FOSB plasmid; (D) The bubble diagrams presenting the top 10 biological processes significantly enriched by the GO enrichment analyses for the three up-regulated DEGs sets (P < 0.05) in H1299 cells expressing p53-Null, p53-WT, and p53-R248Q upon the transfection of the FOSB plasmid; (E) The Venn diagram exhibiting the candidate transcriptional targets of FOSB in H1299 cells expressing p53-Null, p53-WT, and p53-R248Q, respectively; (F) Effects of FOSB overexpression on the mRNA expression levels of its candidate transcriptional targets in H1299 cells expressing p53-Null, detected by the RT-qPCR; (G) Effects of FOSB overexpression on the mRNA expression levels of its candidate transcriptional targets in H1299 and A549 cells expressing p53-WT, detected by the RT-qPCR; (H) Effects of FOSB overexpression on the mRNA expression levels of its candidate transcriptional targets in H1299 and PC-9 cells expressing p53-R248Q, detected by the RT-qPCR. * P < 0.05, ** P < 0.01, *** P < 0.001