Fig. 7
Downregulation of PDLIM2 in lung cancer is associated with increased HIF-1α expression. A, B LLC and A549 lung cancer cells were treated with 400 μM H2O2, 5 mM succinate, and 20 mM dimethyl succinate (DMS) for 6 h. Protein level of HIF-1α and PHD2 was measured by immunoblotting. C, D Protein level of HIF-1α and PHD2 was measured by immunoblotting in PDLIM2 knockdown LLC and A549 cells. E UALCAN database provided data showing HIF-1α expression in normal and different stages of lung adenocarcinoma from patients in the TCGA dataset, with corresponding p-values (*** p < 0.001). F HIF-1α expression was analyzed in TissueScan lung cancer cDNA arrays with normal tissue (n = 15) and tumor biopsies (n = 81) across various tumor stages. G Co-expression analysis of PDLIM2 and HIF-1α by using cBioportal for cancer genomics. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the shEmpty group, control, and the normal tissue, respectively