Fig. 3

PDLIM2 downregulation impairs expression of genes for mitochondrial functions. A Gene ontology enrichment analysis of microarray data by comparing shEmpty and shPDLIM2 LLC cells. Analysis focused on changes with -log fold-changes exceeding 2 or falling below -2. B Fold change in the expression of TCA cycle-related genes derived from the mitochondrial term in microarray analysis. The genes encoding subunits of SDH complex are boxed with red color. C, D mRNA expression levels of SDH subunits, TNF-α, IL-1β, and MMP9 were measured by real-time PCR following stable PDLIM2 knockdown in LLC and A549 cells. E, F Protein levels of SDH subunits in PDLIM2 knockdown LLC and A549 cells were measured by immunoblotting. Data represent means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the shEmpty group