Fig. 6

PAX6 promotes NE characteristics via STAT5A. a The heatmap of candidate TFs with significant expressional difference in DU145-shPAX6 cells and DU145-Scramble cells. b Comparisons of STAT5A mRNA expression in CRPC-Adeno vs. NEPC based on the GSE32967 dataset (CRPC-Adeno, n = 8; NEPC, n = 14). c Representative IHC staining of STAT5A in tissues from patient with Primary PCa, CRPC or NEPC (Scale Bar: 100 μm). d ChIP assay of PAX6 binding at the promoter region of STAT5A in LNCaP-PAX6 cells. e Protein expression of PAX6, STAT5A, SYP and NSE in DU145-shPAX6 cells with or without STAT5A overexpression. f Cell proliferation assay in DU145-shPAX6 cells with or without STAT5A overexpression. g Representative image and quantification assay of tumorsphere formation in DU145-shPAX6 cells with or without STAT5A over-expression. h Anatomic tumor images and tumor weight analysis of DU145-shPAX6 cells inoculated xenografts with or without STAT5A overexpression (n = 6). i Tumor volume analysis of DU145-Scramble, DU145-shPAX6 or DU145-shPAX6 + STAT5A cells inoculated xenografts respectively (n = 6). j Tumor weights analysis of DU145-shPAX6 and DU145-shPAX6 + STAT5A cells inoculated xenografts respectively (n = 6). k Representative staining H&E and IHC staining of PAX6, Ki67, SYP, NSE, NCAM1 in DU145-shPAX6 and DU145- shPAX6 + STAT5A cells inoculated xenograft samples (Scale Bar: 100 μm, with the boxed region enlarged and shown on the left, n = 6). All the experiments were repeated for three times. Data represents the mean ± SD. ns: no significance, *p < 0.05, ***p < 0.001