Fig. 1
ILK promotes the CSC phenotype and correlates with poor PFS and metastasis in OC patients. A PFS curves for ILK mRNA expression in HGSOC patients generated using the KM plotter (HR = 1.63 and P = 7.3e-05). B OS curves for ILK mRNA expression in HGSOC patients generated using the KM plotter (HR = 1.47 and P = 0.004). C TNMplot showing a significant increase of ILK expression in metastatic samples compared to primary tumor (P = 2.53e-03). D Correlation between ALDH1A1 and ILK mRNA expression levels (Spearman r = 0.18, P = 8.48e-03, Q = 0.0192) in the OC TCGA datasets obtained from cBioPortal. E ALDH1A1 and ILK mRNA expression levels show a tendency toward co-occurrence (Log2 odds ratio = 1.679, P = 0.04, Q = 0.04) in the OC TCGA datasets obtained from cBioPortal. F PFS curves for ALDH1A1 and ILK mRNA expression in HGSOC patients generated using the KM plotter (HR = 1.52 and P = 0.0014). G OS curves for ALDH1A1 and ILK mRNA expression in HGSOC patients generated using the KM plotter (HR = 1.42 and P = 0.018). H WB for p-ILKSer246, ILK, ALDH1A1, and GAPDH in OVCAR-3, COV362, and HEY-A8 monolayers (m) vs. spheroids (s) (left panel). Densitometry quantifies ALDH1A1 and p-ILKSer246/ILK ratio (right panel) (N = 3; ****P < 0.0001). I WB for p-ILKSer246, ILK, ALDH1A1, and GAPDH in UHB-1912–02, UHB-2012–06, and UHB-2101–09 primary OC cells grown as spheroids vs. monolayers (m) (left panel). Densitometry quantifies ALDH1A1 and p-ILKSer246/ILK ratio (right panel) (N = 3; **P < 0.01 and ****P < 0.0001). J ILK mRNA levels measured by qRT-PCR in ALDH+/CD133+ vs ALDH−/CD133− isolated from UHB-2012–06 primary human OC cells, HEY-A8, and OVCAR-3 cells (N = 3; **P < 0.01). K P-ILKSer246 expression levels detected by IHC in human serous ovarian tumors, metastatic, and normal ovarian epithelium included on a TMA (left). Representative images are shown. Scale bar, 200 µm and 500 µm. Quantification of p-ILKSer246 protein levels by intensity with H-score in OC TMA analysis (right). Error bars show mean (± STD) (N = 3; *P < 0.05, **P < 0.01, ****P < 0.0001)