Fig. 5

TSPAN18 promotes migration and invasion of PCa cells through STIM1-Ca²⁺ signal pathway in vitro. a-b Representative images (a) and histogram analysis (b) of wound-healing assay using DU145 and PC-3 cells treated as indicated. c-d Representative images (c) and histogram analysis (d) of migration assays using DU145 and PC-3 cells treated as indicated. e-f Representative images (e) and histogram analysis (f) of invasion assay using DU145 and PC-3 cells treated as indicated. g-h Representative images (g) and histogram analysis (h) of focal adhesions visualized by Vinculin staining within indicated cells. Scale bar, 10 μm. i the protein levels of N-cadherin and E-cadherin were analyzed by Western Blot (WB) within DU145 and PC-3 cells treated as indicated. j The immunofluorescence staining of E-cadherin within DU145 and PC-3 cells transfected with indicated siRNAs. Scale bar, 20 μm. k Histogram analysis of migrated (left) and invaded (right) TSPAN18-overexpressing DU145 and PC-3 cells transfected with indicated siRNAs. l Histogram analysis of migrated (left) and invaded (right) TSPAN18-overexpressing DU145 and PC-3 cells treated with SKF96365 or DMSO. m WB analysis of N-cadherin and E-cadherin within TSPAN18-overexpressing DU145 and PC-3 cells transfected with indicated siRNAs. n WB analysis of N-cadherin and E-cadherin within TSPAN18-overexpressing DU145 and PC-3 cells treated with SKF96365 or DMSO. The values are expressed as the mean ± s.d. of three independent experiments. **p<0.01, ***p < 0.001, Student’s t test or ANOVA with post hoc test