Fig. 4
TSPAN18 activates Ca2+ signal pathway via STIM1-dependent manner. a-b After 20 minutes incubation with Ca2+-sensitive dye Fluo-4, the cytosolic Ca2+ level within indicated cells were measured by confocal laser scanning microscope. The representative time-lapse images (a) and quantification (b) of mean cytosolic Ca2+ level within per high-power field during measurement. Scale bar, 20 μm. RFU: Relative fluorescence unit. c Volcano plots displaying the genes expression change in DU145 transfected with TSPAN18-siRNAs, compared with control-siRNA. Differential expression values were plotted against p-value; Red dots represent the up-regulated genes and blue dots represented down-regulated genes. siT18: siTSPAN18. d A bubble chart exhibiting the enrichment of differentially expressed genes in signaling pathways. Size and color of the bubble represent the amount of differentially expressed genes enriched in pathways and their enrichment significance, respectively. e Gene set enrichment analysis (GSEA) plots of Ca2+ signal pathway in RNA-Seq data upon silencing of TSPAN18 in DU145 cells. NES normalized enrichment score. p-values in panel. f DU145 cells were transfected with indicated siRNAs for 48h, followed by loaded with Fluo-4, and the mean cytosolic Ca2+ level within per high-power field were measured by confocal laser scanning microscope