Fig. 5

Hyperglycemia promotes SCD1 expression as well as migration and invasion of CRC cells. a, b) IPGTT (a) and ITT (b) of chow-fed and HFD-fed mice. c Representative Western blot and quantified results of SCD1 and ChREBP in colon tissues from chow and HFD fed mice. d, e HCT116 cells treated with glucose for 24 h and expression levels of SCD1 and ChREBP were determined by Real Time-PCR (d) and Western blot (e). f Transwell assay of HCT116 cells treated with 0 mM (G0), 5.5 mM (G5.5), 11 mM (G11) or 25 mM (G25) glucose. The scale bar is 100 μm. g, h) Histograms show the numbers of migrated cells (g) and invasive cells (h) under different glucose conditions